PRODUCT SPECIFICATIONS

ITEM# 1081:  Recombinant HIV-1 ADA Envelope Glycoprotein gp120 ( Baculovirus)                     

 Concentration:                See vial                                 

   Mass/vial                          See vial                                                                              Diluent                                 PBS                                            

   Purity                                 >98%                                                                       

   Stabilizer                           None                                                                        

   Preservative:                    None

  Storage:                              -75oC

  Physical State:                  Frozen Liquid

  Stability:                            At least 24 months at -75oC.           

DESCRIPTION:   Gene cloned from M cell-tropic HIV-1 ADA strain and the full length  recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression  System.

 PURIFICATION: This protein is purified by immuno-affinity chromatography to >95% purity as    determined by SDS-PAGE, reduced.

 SPECIFICITY:   This protein binds to murine monoclonal antibodies of defined epitope specificity and   HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.

 BIOLOGICAL ACTIVITY:  This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as  determined by CD4/gp120/Anti gp120 mAb-peroxidase capture ELISA. This protein   activates human monocytic cells (CD4+, CD4-), in vitro, as measured by RNA  synthesis during G0 to G1 transition phase of antigen-binding competent cells.

 APPLICATIONS

  CD4 Binding, Cell activation, In-Vitro Diagnostics.

INSTRUCTIONS FOR USE

 Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications

 ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.

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