Product Specifications:

Item# 2001: Recombinant HIV-2 (ROD) Glycoprotein gp105

Concentration: See vial

Mass/vial: 100ug 

 Volume/vial: See vial 

 Diluent: PBS  

 Purity: >90% 

Stabilizer: None 

 Preservative: None

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: At least 12 months at -75°C.

 Application: Human Diagnostics,Drug Screening,CD4 Binding,Vaccines,Immunization,T-Cell Activation.

 Description: Full length Recombinant HIV-2 (ROD) gp105 glycoprotein produced in the Baculovirus Expression System.

 Purification: This protein is purified by immunoaffinity chromatography to >90% purity as determined by SDS-PAGE, reduced.

 Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-2 human serum polyclonal antibodies in ELISA and Western ELISA.

 Biological Activity: This protein binds to human T-cell receptor CD4, albeit with lower affinity compared to HIV-1 gp120, in ELISA and Western ELISA as determined by CD4/gp105/Anti- gp105 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.

 Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.

 Item#: 2001-P Peroxidase-Conjugated rgp105 HIV-2 (ROD)

 Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.

Item#: 2001-G Colloidal gold-Conjugated rgp105 HIV-2 (ROD)

Application: Human diagnostics, CD4 binding, Drug screening, FACS analysis.

Item#: 2001-F Flourescene-Conjugated rgp105 HIV-2 (ROD)

Application: FACS analysis, Histocytology.

Item#: 2001-M Magnetically-Coupled rgp105 HIV-2 (ROD)

Application: Human diagnostics, Human B and T-cell panning.