PRODUCT SPECIFICATIONS

ITEM# 4003:  Recombinant HCV 20kD Core Protein (Pichia)

SPECIFICATIONS APPLICATIONS

 Concentration: 500ug/ml   In-Vitro HCV Diagnostics

  Mass/vial: 100ug   T-Cell Activation

  Volume/vial: 200ul  

  Diluent: 20mM Citrate-Phosphate, pH6.0, 0.5M Urea  

  Purity : >90%    

  Stabilizer: None    

  Preservative: None  

  Storage: -75o C

  Stability: At least 6 months at -75o C

DESCRIPTION:   Full length HCV p22 capsid his-tag protein expressed in the Pichia pastori Expression System.

PURIFICATION:  HCV core fusion protein is purified by Ni-affinity chromatography methods to >   90% purity as determined by SDS-PAGE, reduced.

SPECIFICITY:       This protein binds to murine monoclonal antibodies of defined epitope specificity   and HCV converted human serum polyclonal antibodies in ELISA and Western   ELISA.

BIOLOGICAL ACTIVITY:   Not determined.

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 50 to 100-fold in suitable buffer for solid phase antigen-antibody titration assays.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

HCV core protein shows relatively strong biding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.