PRODUCT SPECIFICATIONS

ITEM# 5009:  Recombinant SIV gp41 Envelope Protein (E.coli)

  SPECIFICATIONS                                                              

  Concentration:                 See vial                                                   

  Mass/vial:                          0.1mg                                                           

  Volume/vial:                     see vial                                                            

  Diluent:                          50 mM Tris, 0.1M NaCl, 2mM DDT, pH8     

  Purity:                                >98%                       

  Stabilizer:                          None                                                                        

  Preservative:                     None                                                                     

  Storage:                              -75oC

  Physical State:                  Frozen Liquid

  Stability:                            At least 24 months at -75 oC.                          

DESCRIPTION: Truncated Recombinant SIV gp41 envelope protein produced in the E.coli Expression System. Truncated gp41 protein mol.wt: 28kD.

Applications :     In- Vitro Diagnostics,Immunization, Elisa/Western Blots.

PURIFICATION:   This protein was purified by solvent extraction and by preparative  electrophoresis to >98% purity as determined by SDS-PAGE, reduced.                                                                                                        

SPECIFICITY: This protein binds to HIV-2 converted human serum polyclonal antibodies in ELISA  and Western  Elisa.

 BIOLOGICAL ACTIVITY:  Not done

 APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

 ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.

Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard

ELISA conditions.