Product Specifications:

Item# 30080: Recombinant Dengue Antigen D3 Envelope (E.coli))

Concentration: See vial 

Mass/vial: 1mg 

Diluent: 50mM NaCl, 50mM Tris, pH8.5, 0.02%SDS

Purity: >95%

Stabilizer: None

Preservative: None

Storage: -75⁰C

Physical State: Frozen Liquid

Stability: At least 36 months at -75⁰C

Applications: Elisa/Western Elisa, Diagnostics

Description: Synthetic Dengue virus D3 Envelope DNA sequence, amino acid residues 1-416 , expressed in the E.coli Expression System.

Purification: This protein is purified by Ni affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. MW: approx. 75kD.

Specificity: This protein binds to murine monoclonal antibodies- structural/non-structural epitopes, and to Dengue virus converted human sera as determined by Elisa, Western Blots and by one-step lateral flow diagnostic testing.

Application and Instructions for use

Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.

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