Product Specifications:

Item# 30070: Recombinant Dengue D2 Envelope Protein (E.coli)    

Concentration: See vial

Mass/vial : See vial 

Diluent : 50mMTris, pH8, 10mM NaCl

Purity : >95%

Stabilizer : None

Preservative: None

Storage: -75⁰C

Physical State:Frozen Liquid

Stability: At least 36 months at -75⁰C.

Applications:  Elisa/Western Elisa, Diagnostics

Description: Synthetic Dengue virus D2 Envelope DNA sequence, amino acid residues 1-428, expressed in the E.coli Expression System.

Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to  >95% purity as determined by SDS-PAGE, reduced. 

Molecular Weight: approx. 49kD.

Specificity: This protein shows strong reactivity with Dengue virus converted human serum antibodies as determined by Elisa and Western Blot assays. Useful for Dengue serum IgG/IgM detection.

Application and Instructions for use

Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.

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