Product Specifications:

Item# 63001: Recombinant West Nile Envelope E Protein (E.coli)

Concentration: See vial

Mass/vial: See vial

Diluent: 50mM Tris, 0.02% SDS, pH8

Purity: >95%

Stabilizer: None

Preservative: None

Storage: -75°C

Physical State: Frozen Liquid

Applications: ELISA, Western Blot, West Nile Diagnostics

Description: Synthetic West Nile full length envelope E protein DNA sequence, expressed as a his-tag protein in the E.coli Expression System.

Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.

Molecular Weight: approx. 56.1kD

Specificity: This protein binds to murine monoclonal antibodies- structural epitopes, and to West Nile virus converted horse sera as determined by Elisa and Western blot assays.

Biological Activity: Not Determined.

Application and Instructions for use: 

Recommended concentrations for use are approximate values. Elisa is performed in 1-2uglml protein range for titeringvirus converted horse serum polyclonal antibodies.