Product# 1108:  Murine HIV-1 anti-nef Monoclonal Antibody

SPECIFICATIONS                                      APPLICATIONS

  Antibody Class: IgG1                                ELISA, Western ELISA

  Concentration: See vial                             Immunoprecipitation

  Mass/vial: 100µg                                       Immunohistochemistry

  Purity: >95%                                             Diagnostics

  Stabilizer: None

  Preservative: None

  Physical State: Frozen Liquid

  Storage: -75oC

  Stability: At least 12 months at -75oC

DESCRIPTION: High affinity murine anti-nef HIV-1 (LAV) mAb.

SPECIFICITY: Binds to native and recombinant nef in ELISA and Western ELISA.

BIOLOGICAL ACTIVITY: Not Determined.

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays.  Western ELISA requires 1-5µg of mAb/ml.