PRODUCT SPECIFICATION

Product# 3504:  Human Anti-gp160 HIV-1 Monoclonal Antibody

  SPECIFICATIONS                                             APPLICATIONS

  Antibody Class: IgG                                         ELISA, Western ELISA

  Concentration: See vial                                    Immunoprecipitation

  Mass/vial: 100µg                                              Immunohistochemistry

  Purity: >95%  HIV-1 Neutralization

  Stabilizer: None  

  Preservative: None  

  Physical State: Frozen Liquid

  Storage: -75oC

  Stability: At least 2 months at -750C.

DESCRIPTION: High affinity Human anti-gp160 HIV-1 mAb.

SPECIFICITY: Binds to CD4 binding region of gp160.  

BIOLOGICAL ACTIVITY: Neutralizes HIV-1 IIIB at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 IIIB infected cells and co-cultures of infected PBLs.

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays.  Western ELISA requires 1-5µg of mAb/ml. Neutralization assays are performed in 1-10µg/ml range depending on virus TCID.

CAUTION:  Avoid use of needles and ensure proper laboratory safety precautions for the use of this product.