Product Specifications:

Item#1001-G: rgp120 HIV-1 IIIB Conjugated to Colloidal Gold 

Concentration: See vial 

Volume/vial: 1ml Gold Conjugate

Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3 

Storage: 4°C

Stability: At least 3 months at 4°C Minimum

Dilution: 1 : 50

Application: Dot Blot Assay-CD4 Binding, Dot Blot Assay-Anti-gp120 Antibody Binding, Immunohistochemistry. 

Description: rgp120 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).

Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.

Specificity: Gold conjugated rgp120 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays.

 Biological Activity: Not determined.

 Application and Instructions for use:

 Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system.

2. Immunohistochemical studies may be performed in 1 : 50 dilution range.

 Related Items:

Item#: 1001-P Peroxidase-Conjugated rgp120 HIV-1 IIIB

Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.

 Item#: 1001-F Flourescene-Conjugated rgp120 HIV-1 IIIB

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 Item#: 1001-M Magnetically-Coupled rgp120 HIV-1 IIIB

 Application: Human diagnostics, Human B and T-cell panning