Product Specifications: Item # 1106-F: Fluorescene Murine Anti-p66 (RT) HIV-1 mAb IgG Mass/vial: Approximately 50µg IgG Vol/vial: 50ul Diluent: 10mM Tris, pH 8.0 Stabilizer: None Preservative: None Storage: -75°C F/P Ratio: 3 - 4 Stability: At least 6 months at -75°C Minimum Dilution: 1 : 50 Applications: Dot Blots, Immunohistocytology. Description: Fluorescene conjugated Murine Anti-p66 HIV-1 mAb. Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography. Specificity: This fluorescene conjugated mAb binds to native and recombinant HIV-1 p66 as determined by dot blot and Western blot assays. Biological Activity: None Application and Instruction for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dot Blot assays with purified p66 may be performed in 1 : 200 dilution range. P66 may be detected in viral lysates by direct fluorescent assays. |