Product Specifications:

Item # 1108-F: R- Fluorescene Murine anti-nef HIV-1Monoclonal Antibody

 Antibody class:  IgG1

 Vol/vial: 50ul

 Diluent: 10mM Tris, pH 8.0                                  

 Stabilizer: None                                                       

 Preservative: None                                                       

 Storage: -75°C                                                                             

 F/P Ratio: 3 - 4

 Physical State: Frozen Liquid

 Stability: At least 6 months at -75°C

 Minimum Dilution: 1 : 100

 Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.

 Description: R-Phycoerythin conjugated Murine Anti-vif HIV-1 mAb (clone 15).

 Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.

 Specificity: This R-Phycoerythrin conjugated mAb binds to native and recombinant HIV-1 vif as determined by dot blot and Western blot assays. Detection range: 50-200pg vif.

 Biological Activity: None

Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dot Blot assays with purified vif may be performed in 1 : 200 dilution range. vif may be detected in viral lysates by direct immunofluorescent  assays. R-Phycoerythrin conjugates can be used with three- and four-color fluorescence cell sorting. R-Phycoerythrin is excited with argon ion laser at 488nm and detected at 570nm.