Product Specifications:

Item # 1301-F: Fluorescene Rabbit Anti-gp120 HIV-1 IIIB pAb IgG

Mass/vial: Approx. 50ug IgG

Vol/vial: 100ul

Diluent: 10mM Tris, pH 8.0                                  

Stabilizer: None                                                       

Preservative: None                                                       

Storage: -75°C                                                                             

F/P Ratio: 2 - 4

Physical State: Frozen Liquid

Stability: At least 12 months at -75°C

Minimum Dilution: 1 : 50

Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.

Description: Fluorescene conjugated Rabbit anti-gp120 (HIV-1 IIIB) pAb IgG.

Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.

Specificity: This fluorescene conjugated pAb IgG binds to native and recombinant HIV-1 gp120 in Dot Blot assays and stains cell surfaces in direct immunofluorescence assays.

Biological Activity: None

Application and Instruction for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified gp120 may be performed at a minimum dilution of 1 : 100.